Therapeutic effect and mechanism of Qianggu Shengxue Oral Liquid on ovariectomy-induced osteoporosis in rats / 中草药

ABSTRACT

Objective To study the effect of Qianggu Shengxue Oral Liquid (QSOL) on ovariectomy-induced osteoporosis in female rats. Methods 18-weeks old female SD rats were randomly divided into six groups Sham, ovariectomized model group (OVX), three different doses of XLGB-treated groups after OVX (2.7, 5.4, and 10.0 mL/kg), and a positive drug group treated with xian-ling-gu-bao capsule (0.54 g/kg). In addition to the sham operation, the other groups were subjected to bilateral ovariectomy to establish osteoporosis model. Three days after the ovariectomized operation, rats were orally treated with drugs once per day for 90 continuous days. On the 45th day and the 90th day after given drugs, blood calcium, C-terminal telopeptides of type Icollagen (CTX-I) in serum, bone calcium, hydroxyproline, bone mineral density (BMD), femur bone, and the fourth lumbar vertebra (LV4) for the fracture load, maximum load and histopathologic examination were detected. Results Compared with the model group, the middle and low dose of QSOL can attenuate the decreases in blood calcium, BMD and bone density, decrease the level of serum CTX-I, and improve bone biomechanical property induced by ovariectomy in female rats; The high dose of QSOL can significantly attenuate these decreases in blood calcium, BMD and bone density, decrease the level of serum CTX-I, and improve bone biomechanical performance. Conclusion QSOL oral liquid can delay disease progress and treat the osteoporosis induced by ovariectomy, and the mechanism is related to the reduction in bone calcium loss and the level of CTX-I.