Comparision of contents of thirteen components in different origins of Rhei Radix et Rhizoma based on principal component analysis / 中草药

ABSTRACT

Objective To establish an HPLC method for the simultaneous determination of thirteen effective components in Rhei Radix et Rhizoma and to assess the quality from different producing areas. Methods The analysis was performed on ZORBAX C18 chromatographic column with ographic mobile phase consisted of methanol-0.1% phosphoric acidwater in a gradient mode. The flow rate was 1.0 mL/min. Gallic acid, catechin, epicatechin, aloe-emodin-8-O-glucopyranoside, rhein-8-O-glucopyranoside, emodin-8-O-glucopyranoside, physcionin-8-O-glucopyranoside, chrysophanol-8-O-glucopyranoside, aloe-emodin, rhein, emodin, chrysophanol and physcionemod were determined at 254 nm, and the results were analyzed by principal component analysis (PCA). Results The linear regression equation of 13 components of Rhei Radix et Rhizoma was established within a certain concentration range. The precision and reproducibility were less than 5.00%, the recovery of the method was 95.54%-103.19%, and the sample was stable within 24 h. The contents of Rhei Radix et Rhizoma in 27 batches were differences. Five principal components were obtained by PCA, the principal component integrated model was between 0.335 and 1.905 and the value of Rhei Radix et Rhizoma in Mianyang of Sichuan Province was the highest. Conclusion A method for the determination of thirteen main components of Rhei Radix et Rhizoma was established. PCA was used to evaluate the quality of Rhei Radix et Rhizoma and the main component model value can be used as the evaluation index of Rhei Radix et Rhizoma.