Identification and fragmentation regularity of polyphenol compounds from Galla Quercina by UPLC-MS/MS / 中草药

ABSTRACT

Objective To explore the fragmentation regularity and identify the polyphenol components in Galla Quercina. Methods Waters Xevo TQD liquid chromatography/mass spectrometer ESI negative mode was used to analysis, mobile phase was 0.02% formic acid-water and acetonitrile for gradient eluent. Data were analyzed by Mass Lynx software. Results Three categories and 15 compounds in Galla Quercina were identified and divided into gallotannins, ellagitannins, and phenolic acids. Gallotannins was cleaved by loss of gallic acyl, C2H2O, CH2O, CO2, and OH, and the gallotannins had the regular loss of gallic acid units (152). Conclusion The results of fragmentation mechanism can be used to detect polyphenol compounds in Galla Quercina and provide a useful reference for the study on the chemical constituents of medicinal plants rich in polyphenols.