Fingerprint and multi-components quantitative determination of Xiaochuan Granula by fused-core column / 中草药

ABSTRACT

Objective To establish an HPLC fingerprint of Xiaochuan Granula (XCG), and to make a quantitative analysis of seven components by fused-core column. Methods Kromasil C18 (150 mm × 4.6 mm, 3.5 μm) was used with the mobile phase of Methanol (A)-acetonitrile (B)-water (C), at a flow rate of 1.0 mL/min. The detection wavelength was set at 210 nm for amygdalin and magnolin, 240 nm for morroniside, loganin, prim-O-glucosylcimifugin, 4'-O-β-glucopyranosyl-5-O-methylvisamminol, schizandrin, and fingerprint; And the column temperature was maintained at 40 ℃. Common peaks had been identified by UPLC-Q-TOF/MS and standard compounds. Results The fingerprint chromatography included 20 mutual peaks, and the similarity was more than 0.90. Eleven chemical components were identified by UPLC-Q-TOF/MS and standard compounds, which were 3-morroniside, oxypaeoniflorin, 5-loganin, 6-prim-O-glucosylcimifugin, 9-4'-O-β-glucopyranosyl-5-O-methylvisamminol, 11-magnolin, 15-schisandrin, 16-schisandrol B, 18-schisantherin A, 19-deoxyschizandrin, and 20-γ-schizandrin B. Moreover, seven active components (morroniside, oxypaeoniflorin, loganin, prim-O-glucosylcimifugin, 4'-O-β-glucopyranosyl-5-O-methylvisamminol,magnolin, and schisandrin) were quantified and the average recovery rates ranged from 97.3% to 103.8% with RSDs less than 2.0%; Seven components in 10 batches samples were morroniside 0.51-0.69 mg/g, amygdalin 5.01-5.95 mg/g, loganin 1.02-1.33 mg/g, prim-O-glucosylcimifugin 0.35-0.45 mg/g, 4'-O-β-glucopyranosyl-5-O-methylvisamminol 0.45-0.55 mg/g, magnolin 0.38-0.48 mg/g, schisandrin 0.89-1.08 mg/g, respectively, and RSD of each component was less than 11.0%. Conclusion The method for establishing HPLC fingerprint and quantitative analysis of seven components is rapid, simple, and accurate, and can be used for the quality control of XCG.