Transcription factor analysis of safflower based on transcriptome / 中草药

ABSTRACT

Objective This research aimed to analyze transcription factors (TFs) and their expression level in different tissues of Carthamus tinctorius. Methods Seed, leaf and petal transcriptome were analyzed by Solexa sequencing and assembled into Unigene. TFs were obtained according to Nr database. Gene expression, conservative Motif and evolutionary tree of MYB family were analyzed. Results A total of 731 transcription factors belong to 42 transcription factor family were obtained. Furthermore, MYB expressed differently in corolla when contrast to seed and leaf. There are three conserved protein domains in MYB family and MYB family was clustered into two subfamilies based on phylogenetic tree results. Conclusion TF families of MADs-box, WRKY, MYB and AP2 were differentially expressed significantly in various tissue samples and MYB TF family are conserved.