Genetic diversity of Trichosanthis Fructus for seeds cultivating / 中草药

ABSTRACT

Objective To reveal the genetic diversity of cultivating resources of Trichosanthes sp. from different areas. Methods The genetic background of 11 populations (including 30 samples) of Trichosanthis Fructus were researched by both ITS and SRAP molecular markers. The software mega 5.0 and NTSY-pc 2.1 were taken to analysis the ITS sequences and SRAP polymorphic bands data respectively. Results There are obvious mutation in ITS sequences among T. kirilowii and T. rosthornii, ITS sequences could authenticate the different resources in some extent. 165 SRAP bands were amplified by using the optimized 15 primer pairs, and 136 were polymorphic bands, the polymorphic rate was up to 82.4%. The samples were separated into two clusters at the genetic similarity coefficient of 0.18, one cluster gathered the samples of Yuelou Num.8 (T. kirilowii) and the other cluster further separated into three sub-clusters at the coefficient of 0.90, which were mainly T. rosthornii. Conclusion There are several species of Trichosanthes have been used to harvest the seeds in China, and the major one is T. rosthornii. The cultivating resources of T. rosthornii have little difference in genetic backgrounds.