Effects of intestinal flora imbalance on pharmacokinetic parameters of baicalin and baicalein in rats / 中草药

ABSTRACT

Objective To study the effects of intestinal flora imbalance after ig administration on pharmacokinetic parameters of baicalin or baicalein in rats. Methods The SD rats were divided into four groups including baicalin group, antibiotic + baicalin group, baicalein group, and antibiotic + baicalein group. Traditional antibiotic medicine lincomyci were used to induce rats gut micro dysbiosis for 5 d. The pharmacokinetics of related technologies and methods were used, and the rats were administered with Moore dose (370 μmol/kg) of baicalin and baicalein. The plasma samples were collected at different time points within 20 h, and the concentration of baicalin was determined with LC-MS/MS, and the curve was drawn. The data were analyzed by DAS.2.2 software, comparison of baicalin and baicalein in animal behavior changes of rats in normal rats and antibiotics. Results The results showed that the baicalein was metabolized entirely and the metabolite baicalin was detected mainly in rat plasma after oral administration of baicalein. These results showed that the Cmax [ (7.80 ± 5.52) mg/L] of baicalin in rats in antibiotic + baicalin group was decreased compared with that with baicalin alone [Cmax (16.35 ± 9.48) mg/L]. Meanwhile, the AUC0~t of baicalin in antibiotic + baicalin group was 32.60 ± 18.88 mg∙h/L which was significantly lower than that in rats with baicalin alone [ (75.16 ± 48.40) mg∙h/L]. These results showed that the Cmax [ (10.28 ± 5.57) mg/L] of baicalin in rats of antibiotic + baicalein group was decreased compared with that with baicalein alone [Cmax (60.39 ± 56.32) mg/L]. Meanwhile, the AUC0~t of baicalin in antibiotic + baicalein group was (71.67 ± 54.49) mg∙h/L which was significantly lower than that in rats with baicalein alone [ (212.51 ± 101.25) mg∙h/L]. Conclusion Baicalin and baicalein showed bad pharmacokinetic behavior in rats when the rat intestinal flora is imbalance.