Molecular cloning, sequence identification, and expression analysis of HMGR gene in Houttuynia cordata / 中草药

ABSTRACT

Objective To clone and analyze the expression difference sequence of 3-hydroxy-3-methylglutaryl coenzyme A redutase (HMGR) gene from Houttuynia cordata. Methods The sequence of HMGR was cloned from H. cordata by RT-PCR strategy. The physical and chemical properties, secondary structure, and three-dimensional structure of the HMGR protein were forecasted and analyzed, and its structure and function were predicted. And the different expression of HMGR gene in rhizome, stems, leaves, and flowers were analyzed by fluorescent quantitative PCR. Results The cDNA contained a 1 626 bp open reading frame and encoded a predicted protein of 541 amino acids. Two transmembrane regions and no signal peptide were present in HMGR. Relative real-time PCR analysis indicated that HMGR showed the highest transcript abundance in the flowers, and the lowest levels in the rhizomes. Conclusion This study cloned and expression analyzed HMGR gene from H. cordata for the first time. The result will provide a foundation for exploring the mechanism of terpenoid biosynthesis in H. cordata.