Study on UPLC fingerprint of Cassiae Semen from two species / 中草药

ABSTRACT

Objective To establish a UPLC fingerprint of Cassiae Semen from two species which is expected to provide standard for the quality control and evaluation. Methods Chromatography conditions were Agilent ZORBAX EP C18 (100 mm × 3.0 mm, 1.8 μm) column with gradient mobile phase of acetonitrile-0.01% formic acid solution. UV detection wavelength was 285 nm, the flow rate was 0.4 mL/min, the column temperature was 30 ℃ and the sample quantity was 1 μL. Results The common mode of UPLC fingerprint of Cassiae Semen was established firstly. There were 20 common peaks had been calibrated by similarity evaluation. Seven peaks had been identified by comparing retention time with UV spectrum analysis. The similarity evaluation of 20 batches samples of Cassiae Semen was more than 0.9. Twenty batches of Cassiae Semen could be divided into four grades when the sum of squared Euclidean distance is 5 become the result of Cluster Analysis. Principal component analysis got three principal components through dimension reduction. The results of principal component scores agree with the clustering analysis. By fitting the load factor model of the first principal component, characteristic peak such as rubrofusarin-6-O-β-gentiobioside were filtered to appraise Cassiae Semen's quality. Conclusion The contents of same chemical compositions between Cassia obtusifolia and C. tora are different. UPLC fingerprint can be used in Cassiae Semen's quality control and evaluation.