Chemical constituents of Ajuga ovalifolia var. calantha / 中草药

ABSTRACT

Objective To investigate the chemical constituents of Ajuga ovalifolia var. calantha. Methods The 95% ethanol extract from the whole herb of A. ovalifolia var. calantha was separated and purified by column chromatography over silica gel, Sephadex LH-20, ODS, and RP-HPLC. Their chemical structures were identified on the basis of physicochemical characteristics and spectral analyses. Results Thirteen compounds were isolated and identified as (16S)-12,16-epoxy-11,14-dihydroxy-17 (15→16)-abeo-abieta- 8,11,13-trien-7-one (1), ajuforrestin B (2), ajudecumin A (3), 14,15-dihydroajugapitin (4), cyasterone (5), β-sitosterol (6), acacetin (7), apigenin (8), luteolin (9), scopoletin (10), isoscopoletin (11), 4-hydroxy-3-methoxy-benzaldehyde (12), and acetovanillon (13). Conclusion Compounds 1 and 10-13 are reported from the genus Ajuga Linn., and compounds 2-5 and 7 are isolated from this plant for the first time. Single crystal X-ray diffraction experiment is carried out for compound 1, and the crystal structure data of single crystal X-ray diffraction of compound 1 is obtained for the first time.