Study on fingerprint of polysaccharides from Jinshuibao Capsule / 中草药

ABSTRACT

Objective: To establish a polysaccharide fingerprint analysis method for identifying Jinshuibao Capsule. Methods: Polysaccharide from fermentation Cordyceps powder in Jinshuibao Capsule was extracted by boiling water, hydrolyzed by acid, and precolumn derivatization method for 1-phenyl-3-meth yl-5-pyrazolone (PMP), analyzed by HPLC. The chromatographic conditions were Phenomenex OOG-4252-EO C18 column (250 mm × 4.6 mm, 5 μm), eluting with acetonitrile-0.05 mol/L phosphate buffer salt gradient, and the detection wavelength was 250 nm. The column temperature was 30 ℃. Results: Eleven monosaccharide components that made up the fermentation Cordyceps powder were identified and established the fingerprint of fermentation Cordyceps powder polysaccharide. Ten batches of monosaccharide components in Jinshuibao Capsule were analyzed by fingerprints. The similarity was greater than 0.99 with no significant difference. Conclusion: The fingerprints of fermentation Cordyceps powder were simple, specific and reproducible, and the quality of Jinshuibao Capsule is evaluated objectively.