Effect of borneol on puerarin through in vitro blood-brain barrier / 中草药

ABSTRACT

Objective: To investigate the effect of borneol on puerarin through in vitro blood-brain barrier (BBB) model and to discuss the main pathway of borneol to promote the opening of BBB. Methods: MTT assay was conducted to investigate the toxic effects of borneol and puerarin with different concentration on the cells and to screen the concentration of tested drug. In vitro model of BBB was used to observe the effect of borneol on the opening of tight junction and the effect of borneol on puerarin through BBB. Results: The experimental drug concentration of borneol and puerarin was both 50 μmol/L by MTT experiment. There was no significant change in transepithelial electrical resistance (TEER) before administration and 24 h after administration, and the permeation rate of puerarin group and borneol + puerarin group were (59.96 ± 5.90)% and (106.80 ± 2.73)%, respectively, with significant difference between two groups. Conclusion: Borneol combined with puerarin can promote its permeation rate to a certain extent, but its mechanism needs to be further explored by cell-related tight junction proteins level and adenosine receptor signaling pathway.