Pharmacological study on Duzhong Butiansu Tablet in improvement of male growth and reproductive capacity / 中草药

ABSTRACT

Objective: To develop Duzhong Butiansu Tablet (DBT) for the second time and add the effect on male infertility, so as to provide an experimental basis for the treatment of male infertility. Methods: The male mice were randomly divided into five groups high, medium, and low dose groups of DBT, control group, and positive drug group. The immune and gonadal organ index, anti-fatigue effect and fecuudity of each group were observed. The male SD rats were randomly divided into six groups high, medium, and low dose groups of DBT, control group, model group, and positive drug group. The changes of organ index, testicular pathological structure, levels of serum sex hormones and effects of serum oxidative stress and fecuudity were measured. The morphological structure of testes was observed by HE staining. Results: By improving the immune function and sex organ index of young male mice, DBT had the anti-fatigue effect on extending the swimming survival time, which would promote the increase of pregnancy rate of female mice. By increasing the weight of model rats, DBT could increase sexual organs and kidney indexes, improve contents of serum sex hormones FSH, LH, T, and E2 and reduce content of MDA in serum, and has anti-oxidant effect of increasing serum SOD and GSH-Px levels, and improves the testicular pathological damage, thus contributs to increase the pregnancy rate of female rats and the average litter size and production of anti-rat spermatogenic dysfunction induced by adenine effect. Conclusion: DBT can improve the growth and reproductive capacity to reverse the impairment effects on male animal, and provide experimentalbasis for the clinical research.