Pharmacodynamics research of Compound Jianshen Granules on rats with type 2 diabetic nephropathy / 中草药

ABSTRACT

Objective: To study the pharmacodynamics effect of Compound Jianshen Granules (CJG) on rats with type 2 diabetic nephopathy induced by high-fat and sugar diet combined low dose streptozotocin (STZ), and to investigate its mechanism. Methods: Type 2 diabetic nephropathy animal model was established by feeding with high fat and sugar diet combined with low dose of STZ. FBS, Scr, BUN, GHb, TC, TG, SOD, MDA, etc were measured to study its effects of reducing blood glucose and protecting kidney, data were dealed with SPSS17.0 software and analyzed in statistics, its pharmacodynamic effects were expounded by kidney and pancreas pathological section. MCP-1, ICAM-1, and IL-6 were tested by ELISA to probe into its mechanism tentatively. Results: Compared with the model group, the weight of each drug administration group of CJG entirely rised, and the difference had statistical significance (P < 0.01); The food, water, urine volume, and urine protein content of each drug administration group of CJG decreased, and the difference had statistical significance (P < 0.05, 0.01); The blood glucose level of each drug administration group of CJG decreased, and the difference had statistical significance (P < 0.05, 0.01); The Scr and BUN of each drug administration group of CJG decreased, and the difference had statistical significance (P < 0.05, 0.01); The GHb of each drug administration group of CJG decreased significantly (P < 0.05, 0.01); TC, TG, and MDA of each drug administration group of CJG decreased significantly (P < 0.01); SOD of each drug administration group of CJG increased significantly (P < 0.01); IL-6, MCP-1, and ICAM-1 of each drug administration group of CJG decreased significantly (P < 0.01). Conclusion: FBG and each biochemical criterion and pathological section results demonstrate that CJG have the certain effects of decreasing blood sugar and protecting kidney, moreover traeatment groups have significant difference compared with model groups. ELISA determination results indicate that the action mechanism of CJG is potentially related to MCP-1, ICAM-1, and IL-6 inflammatory pathway.