Effects of different processing methods on content of crude protein and hydrolytic amino acids in Cervi Cornu Pantotrichum / 中草药

ABSTRACT

Objective: To compare the contents of protein and amino acids in different parts of Cervi Cornu Pantotrichum (CCP) with different processing methods, in order to provide a guidance for the processing and comprehensive utilization of CCP. Methods: The techniques of Dumas combustion and cation-exchange chromatography were respectively adopted to determinate the contents of crude protein and 17 amino acids in different parts of CCP with different processing methods, and the difference was compared. Results: The content of crude protein in powder slices of CCP with blood was higher than that of CCP with blood (P 0.05). The crude protein content in wax slices of CCP with freeze-drying processing was less than that with boiling processing (P 0.05). The content of crude protein and TAA in wax slices of CCP is both significantly higher than that in powder slices, bee slices (P 0.05). Conclusion The difference is existed in content of crude protein and amino acids in CCP with different processing methods. The wax silices of CCP are significantly higher than that of powder slices and bee slices. And the difference in powder slices and bee slices is not significantly. The distribution of crude protein and TAA in different parts of CCP with freeze-drying processing is more uniform than CCP with boiling processing.