Quality differences between Mongolian and traditional Chinese medicine Veratri Nigri Radix et Rhizoma by HPLC fingerprint and acute toxicity / 中草药

ABSTRACT

Objective: To evaluate the quality differences between Mongolian medicine and traditional Chinese medicine (TCM) Veratri Nigri Radix et Rhizoma in HPLC fingerprint and acute toxicity, as a fundamental research for quality standazation. Methods: HPLC fingerprint methods were used to analyze the steroid alkaloids and stilbenes of Veratri Nigri Radix et Rhizoma from different origins. A total of 14 batches of crude drugs, including eight Mongolian and six TCM samples, were examined, and their common pattern were established by authoritative software "fingerprint similarity evaluation system". Characteristic peaks in these fingerprints, including 32 steroid alkaloids and 16 stilbenes, were selected to performing cluster analysis, using SPSS 20.0 software with relative peak area as variables. Through the experiments of acute toxicity in mice, their toxicities were examined. Results: HPLC fingerprint methods of steroid alkaloids and stilbenes were established for the quality evaluation of Veratri Nigri Radix et Rhizoma, and their common patterns of Mongolian medicines (RMJ, RMZ) and TCMs (RZJ, RZZ) with high similarities between groups were obtained. In clustering analysis, 14 batches of Veratri Nigri Radix et Rhizoma were divided into two categories distinctly, as Mongolian medicine and TCM groups. Mongolian medicine showed stronger toxicity than TCM with the LD50 values as 0.503 0 and 17.934 3 g/kg, respectively. Conclusion: The quality of Mongolian medicine and TCM Veratri Nigri Radix et Rhizoma in the aspects of efficacy and idications, producing area, chemical composition and acute toxicity were of significant differences. It is necessary and emergency to distinguish the two crude drugs, and establish quality standard of Mongolian medicine Veratri Nigri Radix et Rhizoma.