Antiviral activity and chemical composition of n-butanol extract of isatidis radix / 中草药

ABSTRACT

Objective To investigate the antiviral activity and chemical composition of n-butanol extract of Isatidis Radix. Methods Each component of total n-butanol extracts was detected by solvent and chromatogram, the anti-HSV-1 activities of each component were detected by MTT assay in vitro, each component of total n-butanol extracts was analyzed by CUPLC-Q-TOF-MS, and the data were analyzed by Markview and Excel software to find the difference among different components. According to statistics, the components related to antivirus activities were obtained. Results According to the similarity of chemical composition, 20 components were extracted and separated from n-butanol extract of Isatidis Radix by chromatography, and 10 components had antiviral activity. Forty-four chemical components were detected by UPLC-Q-TOF-MS from n-butanol extracts and their chemical structures were confirmed. Difference analysis of chemical components discovered 15 chemical components such as alkaloids, organic acids, sugars and amino acid derivatives associated with antiviral activity, especially 1-methoxy-3-carbaldehyde, 3-(3’,5’-dimethoxy- 4’-hydroxy)-2-indolinone, 1-methoxy-3-indolinone, 3-(methylsulfinyl) propylglucosinolate, and glucosamine-6-phosphatee in higher levels of the active components, which was worth further study. Conclusion The n-butanol extract of Isatidis Radix mainly contains indole alkaloids, organic acids, nucleosides and amino acids, wherein the single-indole, a sulfur glycosides and sugars and amino acid derivatives are closely related to the antiviral activity.