Genetic diversity of taxus chinensis var. mairei from fujian based on rapd markers / 中草药

ABSTRACT

Objective To reveal the genetic diversity of provenances and populations of Taxus chinensis var. mairei in Fujian province from 23 orgins, providing certain theoretical foundation for the preservation and protection. Methods RAPD molecular marker technology was used to study the genetic diversity of T. chinensis var. mairei in Fujian province. Results The number of observed allele was 1.974 4, the number of effective alleles was 1.603 8, the average value of the Nei’s genetic diversity was 0.351 3 and the Shannon’s information index was 0.522 8. And there existed more accurate information on genetic relationship, diversity among different geographical provenances. The genetic distance of those 23 species ranged from 0.104 to 0.620 7, the genetic coefficients of 23 varieties ranged from 0.401 7 to 0.786 3. The genetic distance of seven T. chinensis var. mairei of different populations ranged from 0.021 0 to 0.379 4, the genetic coefficients ranged from 0.684 3 to 0.979 2. According to Nei’s method calculation of T. chinensis var. mairei, those seven different populations’ genetic diversity was Dst = 0.108 8, differentiation index Gst = 0.369 0, and gene flow coefficient Nm = 0.855 0. 36.9% of the total genetic variation existed among populations, the variation within populations was only 63.1%, and analysis showed high differentiation existed in provenances. Conclusion There is a certain heritable variation among different provenances of T. chinensis var. mairei, but there still exists more accurate information and high similarity coefficients among different populations.