Simultaneous determination of eight components in root of swertia kingii by hplc / 中草药

ABSTRACT

Objective To establish an HPLC method for simultaneously determining eight components, such as sweroside, gentiopicroside, isoorientin, 1,3,7,8-tetrahydroxy-xanthone-1-O-β-D-glucopyranosyl, swertianolin, 1,3,7,8-tetrahydroxy-xanthone, demethylbellidifolin, and 1,5,8-trihydrox-3-methoxyxanthone in the root of Swertia kingii. Methods Chromatographic analysis was achieved on an Agilent Zorbax ODS column (250 mm × 4.6 mm, 5 μm) by gradient elution of acetonitrile-0.5% phosphoric acid in water at 30 ℃. The flow rate was 1.0 mL/min and the detection wavelength was 254 nm. Results The calibration curves of all the eight constituents showed good linearity in a relatively wide concentration range. The linear ranges of swertiamarin, gentiopicroside, isoorientin, 1,3,7,8-tetrahydroxy-xanthone-1-O-β-D-glucopyranosyl, swertianolin, 1,3,7,8-tetrahydroxy-xanthone, demethylbellidifolin, and 1,5,8-trihydrox-3-methoxyxanthone were 3.84-96.00 μg/mL (r = 0.999 6), 3.36-84.00 μg/mL (r = 0.999 2), 5.92-148.00 μg/mL (r = 0.999 8), 4.81-118.00 μg/mL (r = 0.999 2), 4.32-108.00 μg/mL (r = 0.999 3), 4.16-104.00 μg/mL (r = 0.999 2), 5.12-128.00 μg/mL (r = 0.999 4), 4.80-120.00 μg/mL (r = 0.999 6), respectively. The average recoveries were 99.59% (RSD 0.99%), 98.95% (RSD 3.37%), 98.61% (RSD 1.87%), 99.63% (RSD 1.93%), 99.31% (RSD 1.21%), 99.50% (RSD 1.62%), 99.80% (RSD 0.54%), and 98.50% (RSD 1.87%). Conclusion This method is simple, efficient, accurate, and reproducible, and can be used for the determination of eight constituents in root of Swertia kingii. This will promote the comprehensive usage of this plant.