HPLC-UV/ELSD fingerprint of Fangji Huangqi Decoction / 中草药

ABSTRACT

Objective: HPLC-UV/ELSD fingerprint was developed to assign and identify the main characteristic peaks in Fangji Huangqi Decoction (FHD). Methods: High performance liquid chromatography Venusil MP C18 column (250 mm × 4.6 mm, 5 μm) was used with a gradient mobile phase system of acetonitrile-water containing 0.1% formic acid. Ultraviolet detector the detection wavelength was 254 nm; Evaporative light scattering detector The temperature of drift tube was maintained at 110 ℃ and the flow rate of air was 3 L/min. The injection volume was 10 μL; The flow rate was 1 mL/min. The similarities between the HPLC-UV/ELSD fingerprints of 10 batches of FHD extracts were calculated by similarity evaluation software, and the common peaks were assigned and identified simultaneously. Results: The fingerprint of FHD was established. The complementarity among fingerprints of FHD was analyzed, showing a good correlation among 10 batches of FHD. The HPLC-UV fingerprint of FHD including 20 mutual peaks, of which four mutual peaks were from Stephania Tetrandra Radix, nine peaks were from Astragali Radix, eight peaks were from Glycyrrhizae Radix. The HPLC-ELSD fingerprint of FHD included 16 mutual peaks, of which three mutual peaks were from Stephania Tetrandra Radix, seven peaks were from Astragali Radix, seven peaks were from Glycyrrhizae Radix. Nine components were identified by comparison with the reference substance, FHD and adding the reference substance in FHD, which were calycosin-7- glucoside, liquiritin, ononin, liquirtigenin, calycosin, formononetin, astragaloside IV, astragaloside II, and astragaloside I. Conclusion: It is the first time to establish the HPLC-UV/ELSD fingerprint of FHD. The method is simple, accurate, and reproducible, which can be used to characterize the chemical composition information of FHD and provide a scientific evidence for the quality control of FHD.