Detection of 13 anti-diabetic drugs illegally added in health food by HPLC with solid nuclear particle chromatographic column / 中草药

ABSTRACT

Objective: To develop an HPLC method with solid nuclear particle chromatographic column to determine 13 anti-diabetic drugs illegally added in health food. Methods: The analysis was performed by an HPLC system of Waters e2695, with Cortecs-C18 (100 mm × 4.6 mm, 2.7 μm) column. PDA was performed to identify and quantify tolbutamide, glibenclamide, gliclazide, glipizide, gliquidone, glimepiride, rosiglitazone, repaglinide, pioglitazone hydrochloride, metformin, phenformin, buformin hydrochloride, and glibomuride, which were extracted with methanol by ultrasonic. Results: Thirteen linear calibration curves were obtained with r2 ≥ 0.998 7. The precision of the method was shown by RSD (n = 6) ranged from 0.6% to 1.5%. The recoveries were determined at three concentrations and ranged from 95.9% to 104.7%. The ranges of LLOQ were from 1.1 μg/mL to 3.3 μg/mL and the RSDs (n = 9) of inter-day precision were from 0.5% and 1.6%. Seventeen batches in 86 antidiabetic health foods were added the chemicals with positive rate of 20%. The glibenclamide, buformin hydrochloride, rosiglitazone, glimepiride, metformin, and pioglitazone hydrochloride were determined. Conclusion: The method is specific, simple, and fast to detect 13 anti-diabetic drugs illegally added in health food.