Metabolomic study of morroniside on human umbilical vein endothelial cells injury induced by advanced glycation end products / 中草药

ABSTRACT

Objective: To research endogenous metabolites changes of model group on human umbilical vein endothelial cells (HUVECs) injury induced by advanced glycation end products (AGEs) and the accommodation mechanism of morroniside (active components in Cornus officinalis) on the abnormal metabolism. To find potential biomarkers which morroniside protected the injured HUVECs, and to explore mechanisms of morroniside in treatment of HUVECs injury induced by AGEs. Methods: HUVECs were cultivated in vitro, HUVECs injury model was established by the induction of AGEs. Cells were divided into three groups, control group, model group, and treatment group. Cell samples of three groups were determined with UPLC-Q-TOF/MS. Pattern recognition methods including PCA, PLS-DA, and OPLS-DA were applied to analyze multivariate data, and t-test was used in significant statistical analysis. It was used to find out potential biomarkers. Metabolomic feature network graphs were constructed ingenuity pathway analysis (IPA). Results: In pattern recognition, control group, model group, and treatment group could be distinguished better from each other. According to VIP values, 30 ions which had a significant contribution to the classification were screened further, 10 potential metabolic biomarkers were identified qualitatively. These endogenous substances of the cells in treatment group in vivo had varying degrees of recovery. Five metabolic pathways were identified, and a metabolomic feature network of morroniside to protect against HUVECs injury induced by AGEs was constructed. Conclusion: Changed metabolities on HUVECs injury induced by AGEs can be certainly recovered by morroniside, and the treatment effect of morroniside can be connected with the regulation of five related metabolic pathways.