Dynamic changes of development and active ingredients content of male flower of Eucommia ulmoides / 中草药

ABSTRACT

Objective: To establish a method for the determination of six active ingredients in Eucommia ulmoides male flowers, and analyze the dynamic changes of male flower development and active ingredients content of E. ulmoides. Methods: The active ingredients were extracted by the ultrasound-assisted process, the content of total flavonoids was determined by AlCl3 colorimetric method and other six ingredients were determined by HPLC, the samples were separated on a Thermal hypersil gold column (250 mm × 4.6 mm, 5 μm) with gradient elution of methanol-0.5% phosphoric acid at a flow rate of 1.0 mL/min, the UV wavelength was set at 206 nm (0-15 min), 236 nm (15-55 min), and 255 nm (55-100 min). Results: Flower diameter, flower height, fresh weight, dry weight, stamen length and stamen number all reached the maximum at full bloom stage; The contents of total flavonoids, chlorogenic acid and total active ingredients were the highest at the bud stage and the lowest at the initial flowering stage; The content of aucubin showed a tendency of graudal decreasement; The content of geniposidic acid was the lowest at the bud stage and reached the highest at full bloom stage, and decreased at the end of flowering phase; The content of geniposide, isoquercitrin and astragaline were the highest at full bloom stage and the lowest at the initial flowering stage. Conclusion: Flowering stage had a great impact on morphology, yield, active ingredients content and quality of E.ulmoides male flowers, which could provide important information for quality control and product development of E.ulmoides male flowers.