Isolation and identification of metabolites in rat urine after ig administration of calycosin-7-O-β-D-glucopyranoside / 中草药

ABSTRACT

Objective: To study the metabolites from rats urine after ig administration of calycosin-7-O-β-D-glucopyranoside. Methods: The constituents were isolated and purified by preparative liquid chromatographic technique, and the structures were identified by spectroscopic analyses including ESI-MS, NMR, and 2D-NMR. Results: Five metabolites were isolated from rats urine after ig administration of calycosin-7-O-β-D-glucopyranoside. They were identified as calycosin (M1), 3', 4', 7-trihydroxyisoflavone (M2), daidzein (M3), calycosin-3'-O-β-D-glucuronide (M4), and calycosin-3'-O-β-D-glucuronide methyl ester (M5). Conclusion: M5 is a new compound.