Simultaneous determination of 10 active components in Xintong Oral Liquid by HPLC-MS/MS / 中草药

ABSTRACT

Objective: To establish an HPLC-MS/MS method for the simultaneous determination of ferulic acid, tetrahydroxystilbene glucoside, puerarin, calycosin-7-glucoside, naringin, protocatechuic aldehyde, salvianolic acid B, icariin, tanshinone IIA, and synephrine in Xintong Oral Liquid (XOL). Methods: The analysis was performed on Phenomenex Luna-C18 column (250 mm × 4.6 mm, 5 μm) by gradient elution of acetonitrile-mehanol-0.02 mol/L ammonium acetate. The flow rate was 0.9 mL/min. MS conditions electrospray ionization (ESI) and multiple reaction monitoring (MRM) were adopted, the detected peak areas of ion pairs were used for quantitative determination. Results: The linear ranges of ferulic acid, tetrahydroxystilbene glucoside, puerarin, calycosin-7-glucoside, naringin, protocatechuic aldehyde, salvianolic acid B, icariin, tanshinone IIA, and synephrine were 15.22-15 220 (r = 0.999 3), 19.52-19 520 (r = 0.999 4), 25.41-25 410 (r = 0.999 5), 35.27-35 270 (r = 0.999 2), 30.28-30 280 (r = 0.999 4), 50.11-50 110 (r = 0.999 3), 20.33-20 330 (r =0.999 2), 25.22-25 220 (r = 0.999 6), 25.36-25 360 (r = 0.999 3), and 30.29-30 290 ng/mL (r = 0.999 2). The average recoveries (n = 6) were 98.15% (RSD = 1.04%), 101.84% (RSD = 0.98%), 99.86% (RSD = 0.75%), 101.08% (RSD = 0.87%), 100.52% (RSD =1.31%), 100.67% (RSD = 1.27%), 100.46% (RSD =1.64%), 99.43% (RSD =1.48%), 100.81% (RSD = 0.67%), and 98.37% (RSD =1.16%), respectively. The contents of 10 batches of the 10 active components were 0.294-0.319, 0.640-0.665, 4.671-4.699, 0.244-0.264, 2.211-2.231, 0.180-0.201, 0.306-0.324, 1.540-1.564, 0.504-0.522, and 0.809-0.829 mg/mL. Conclusion: This method is simple and rapid, and can be used for the quality control of XOL with satisfactory separation and repeatability.