Study on identification and determination of Lonicerae Japonicae Flos and Lonicerae Flos by UHPLC / 中草药

ABSTRACT

Objective: To explore the differences between chemical constituents of Lonicerae Japonicae Flos and Lonicerae Flos based on the quality and quantity analysis. Methods: The chromatographic separation was carried out on a Luna C18-HST column (100 mm × 3.0 mm, 2.5 μm) with gradient elution kept at 40 ℃. The mobile phases consist of 0.1% formic acid aqueous solution and 0.1% formic acid acetonitrile solution at a flow rate of 1.0 mL/min. The samples were analyzed with a small volume (1 μL) per injection. DAD detection was performed at 327 nm from 0 to 3 min, 350 nm from 3 to 5 min and ELSD detection was performed from 5 to 8 min. Results: Almost all the compounds had good linearities (r = 0.997 6-0.999 9). The excellent recovery rates were 98.71%-100.8% with their RSD from 0.53%-1.87%. Twenty batches of samples obtained from different locations were examined and their chromatographic profiles were compared. Conclusion: The results showed that this method was simple, reliable, and stable, and it could be used to rapidly distinguish Lonicerae Japonicae Flos and Lonicerae Flos with high resolution in a single run within 8 min. Furthermore, the method could accurately determinate chlorogenic acid, luteolin-7-O-glucoside, maranthoidin B, and dipsaccside B.