Spectrum-effect relationship of Salvia miltiorrhiza extract on HIF-1α of retinal Müller cells in AGEs or hypoxia conditions / 中草药

ABSTRACT

Objective: To investigate spectrum-effect relationship of the extract from Salvia miltiorrhiza on HIF-1α of retinal Müller cells in the advanced glycationend products (AGEs) or hypoxia conditions, regarding tanshinone IIA as reference to explore the relationship between pharmacological effects and chemical substances, and to reveal the material basis of S. miltiorrhiza treating diabetic retinopathy (DR). Methods: Fingerprints of different S. miltiorrhiza extract were established by HPLC and characteristic peak's area was recorded. After that, the expression quantity of HIF-1α of retinal Müller cells were tested in the AGEs or hypoxia conditions under the two different conductions S. miltiorrhiza extract or only tanshinone IIA. Gray relational analysis and partial least squares regression analysis (PLSR) were combined to build spectrum-effect relationship. Results: Seventeen characteristic peaks were marked out. Compared with the model set, 10 batches of extracts and tanshinone IIA could lower the expression quantity of HIF-1α. The statistical results showed that the peaks 1, 5, 6, 9, 10, 11, 12, 13, 14, 15, and 16 would inhibit HIF-1α expression, which made great contribution. Conclusion: The 15,16-dihydrotanshinone I, cryptotanshinone, tanshinone I, tanshinone IIA, and other six components might be the main effective components in the ethanol extract from S. miltiorrhiza.