Study on genetic relationship of Paris polyphylla var. yunnanensis and its wild relatives based on infrared spectroscopy / 中草药

ABSTRACT

Objective: The genetic relationship between Paris polyphylla var. yunnanensis and its relatives has been researched using Fourier transform infrared (FT-IR) spectroscopy combined with chemometrics methods in order to provide a theoretical basis for the development and utilization of medicinal plant resources of genus Paris L. Methods: The infrared spectrum information of 50 samples of P. polyphylla var. yunnanensis, P. polyphylla var. alba, P. mairei, P. vietnamensis, and P. axialis var. axialis was collected. The original infrared spectra data were pretreated by automatic baseline correction, automatic smoothing, ordinate normalization, multiplicative scatter correction and second derivative, and analyzed by principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA). Results: The common peaks of 1 653, 1 156, 1 082, 1 021, 925, 851, 759, 572 and 524 cm-1 in original spectra data of 50 samples might be relative to the contents of flavonoids, starches and glycosides. The absorption peaks of 1 535 and 1 369 cm-1 belonged to P. mairei and P. axialis var. axialis, respectively which could be distinguished from other three species. By PCA and PLS-DA, the former one which could accurately distinguish five species of wild genus Paris L. presented a better classification result than the latter. HCA and vector included angle cosine analysis could reflect the genetic relationship of P. polyphylla var. yunnanensis and its wild relatives. P. polyphylla var. alba and P. vietnamensis had closed relationship with P. polyphylla var. yunnanensis while P. mairei and P. axialis var. axialis were relative far. Conclusion: FT-IR spectroscopy combined with chemometrics methods can distinguish different species of genus Paris L. and display the genetic relationship between P. polyphylla var. yunnanensis and its wild relatives, clearly. Furthermore, it could provide a fast and effective method for the study of plant genetic relationships and a theoretical basis for the development and utilization of medicinal plant resources of genus Paris L.