Determination of six flavonoids in Schizonepeta tenuifolia from different areas by HPLC / 中草药

ABSTRACT

Objective: To detect the contents of six kinds of flavonoids in Schizonepeta tenuifolia from different areas, so as to provide a basis for their quality control. Methods: Ultra performance liquid chromatography (UPLC) was used to establish the detection scheme of flavonoids content, and clustering analysis was conducted by the results of content detection. Results: The contents of six kinds of flavonoids in S. tenuifolia from different areas had large differences. The linear ranges were 0.030-0.148 μg for cynaroside, 0.222-1.108 μg for quercetin, 0.357-1.784 μg for hesperidin, 0.058-0.292 μg for luteolin, 0.054-0.269 μg for apigenin, and 0.050-0.247 μg for diosmetin, respectively. The average recovery rate was between 96.32% and 99.97%, RSD < 2.20%; Among this, the Schizonepeta Briq. herb from Anhui was as one class, the herbs from Henan 1 and Guangdong were as one class, and the herbs from Henan 2, Hebei, Yunnan, Sichuan, Hubei, and Gansu were as the other class. Conclusion: The method used for quality control of the multi-index level in this experiment is simple and sensitive; on the other hand, the herbs from Henan 1, Henan 2, Hebei, Yunnan, Sichuan, Hubei, Guangdong and Gansu have a close relationship; They can be used as the same medicinal source.