Effect of solanine on proliferation and apoptosis of U251 cells / 中草药

ABSTRACT

Objective: To investigate the effect of solanine on growth and proliferation of U251 cells. Methods: U251 cells were cultured with different concentration of solanine together. CCK-8 method, wound healing assay, Transwell method, Hochest assay, flow cytometry screening and Western blotting were used to detect inhibitory rate, migration and invasive rates, apoptosis rate and expression level of cell apoptosis-related proteins. Results: CCK-8 assay showed the median inhibition concentration (IC50) of 48 h was 20.05 μg/μL. It was effective that solanine in the concentration range of 5-35 μg/μL could inhibit the growth and proliferation of U251 cells, and played a role in a dose-dependent manner (P < 0.05). Compared with control group, experimental groups can not only inhibit U251 cell migration and invasion in a dose-dependent manner (P < 0.05), but also appear some significant apoptosis characteristics with a concentration dependence. Western blotting assayed that the expression of Bax protein was upregulated, whereas Bcl-2 protein expression was downregulated in experimental groups compared with control group and these changes were dose-dependent. Conclusion: Solanine can inhibit growth and proliferation of U251 cells in effective concentration and in a dose-dependent manner. Solanine can inhibit the invasion and migration of U251 cells. Solanine can induce the apoptosis of U251 cells, and inhibit the proliferation and growth of U251 cells via regulating the expression of Bax and Bcl-2 proteins and affecting Bcl-2/Bax ratio.