Dynamic changes of metabolite accumulation of Polygoni Multifori Radix based on UPLC-Triple TOF-MS/MS / 中草药

ABSTRACT

Objective: To analyze the change of chemical constituents of Polygoni Multifori Radix from different harvest time. Methods: Fifteen batches of Polygoni Multifori Radix samples from different harvest time were determined by UPLC-Triple TOF-MS/MS. Through the analysis of the multistage tandem mass spectrometry, the characteristic peaks were extracted with mass spectrometry data peak matching, peak alignment, and noise filtering. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for data processing. The components were identified according to a mass spectrometry accurate mass and two mass spectrometry fragmentation information, combined with the software of database search and literature. Results: The chemical composition in Polygoni Multifori Radix samples from different harvest time are clearly distinguished. Twenty-four kinds of differential chemical constituents were identified. Among them, there are nine kinds of common differential constituents which presented different changing laws. Conclusion: This study provides the basic data for revealing the dynamic change law of metabolite accumulation of Polygoni Multifori Radix and confirms its optimal harvest period.