Determination of six saponins in Caulophyllum robustum by QAMS / 中草药

ABSTRACT

Objective: To establish a method of quantitative analysis of multi-components by single-marker (QAMS) for simultaneous determining six saponins in Caulophyllum robustum. Methods: Cauloside G was used as the internal reference standard; The relative correction factors of cauloside H, leonticin D, cauloside D, cauloside B, and cauloside C in C. robustum were calculated. To achieve the QAMS, the contents of indicative saponins were calculated by relative correction factors. At the same time, the external standard method was used to detect the contents of six kinds of ingredients in medicinal materials, and feasibility of QAMS was verified. Results: The relative correction factor had good reproducibility, there were no significant differences among the calculated values of multi-evaluation and measured values of the external standard method. Conclusion: In the absence of reference substance situation, QAMS can be used for the quality evaluation of C. robustum, and the method is accurate and reliable.