Determination of five bioactive constituents in wild grown and cultivated Prunella vulgaris by HPLC / 中草药

ABSTRACT

Objective: To compare the contents of phenolic acids and flavonoids in wild grown and cultivated Prunella vulgaris from various habitats, an HPLC method was established for simultaneous determination of five bioactive compounds (rosmarinic acid, salviaflaside, caffeic acid, rutin, and luteolin). Methods: The Agilent 5HC-C18 (250 mm × 4.6 mm, 5 μm) was adopted with a gradient eluent system composed of acetonitrile and 0.1% phosphoric acid aqueous solution at the temperature of 30℃. The flow rate was 1.0 mL/min. The detection wavelength was 280 nm. Independent t-test (t-test), hierarchical clustering analysis (HCA), and correlation analysis were applied to analyzing and evaluating wild grown and cultivated P. vulgaris. Results: The t-test results showed that the contents difference of rosmarinic acid, rutin, caffeic acid, and luteolin had statistical significance (P 0.05). According to the result of HCA, most of the cultivated and wild materials could be differentiated. The result of correlation analysis showed that the ear length has no significant influence on the contents of main compounds in P. vulgaris. Conclusion: The determination method is simple and feasible, and it can be used as one of the quality evaluation method of P. vulgaris.