HPLC fingerprint analysis and content determination of Gleditsiae Spina / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1416-1419, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-852888
ABSTRACT
Objective:
An HPLC method was developed to evaluate the quality of Gleditsiae Spina through three components determination and fingerprint analysis.Methods:
The assay was performed on Waters symmetry C18 (250 mm × 4.6 mm, 5 μm) column. The mobile phase consisted of acetonitrile and 0.1% methanoic acid in water with gradient elution by PDA detection at 338 nm.Results:
Thirteen batches of Gleditsiae Spina were analyzed with the established method. In the fingerprint, 14 common peaks were marked and three of them were determined. The three compounds showed good linearity (r ≥ 0.999 2) in the range of 0.091 3-5.840 0, 0.176 3-11.280 0, and 0.014 0-0.895 0 mg/mL, respectively. The average recoveries were 98.97%-99.66% with RSD < 2.5%.Conclusion:
This method has good repeatability and stability and provides a new method for the quality control of Gleditsiae Spina.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2017
Type:
Article
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