Comparison of cell activity from different parts of Tripterygium wilfordill based on xCELLigence system / 中草药

ABSTRACT

Objective: To compare the bio-activities of different parts of Tripterygium wilfordii from different regions based on RTCA system with HPLC. Methods: Real-time xCELLigence analysis system was established to detect dynamically cellular activity at real-time The seeding density was 2 × 105 cells/mL, the administration time was about 24 h, and the activity of RBL-2H3 cells from T. wilfordii was detected; The fingerprints of different parts of T. wilfordii were established by HPLC and the common peaks were analyzed. Results: The activity of T. wilfordii from Zhejiang was similar at the same site; The root barks and leaves of T. wilfordii had similar biological activity, and the roots and stems had similar activity; The biological activity for RBL-2H3 cells in root barks and buds was significantly higher than that in roots and stems, especially in the root barks, biological activity was the strongest; The fingerprints showed that the components of different parts were similar but the contents were largely different; The inhibitory rate of chloroform extract on RBL-2H3 cells was significantly higher than that of water extract. Conclusion: At the level of cell biology, the velamen of T. wilfordii and other aerial parts possess value of research and medication, which is deserved to further developed and utilized.