Determination of aflatoxins in animal medicines by immunoaffinity column and HPLC-FLD with photochemical derivatization fluorescence detection / 中草药

ABSTRACT

Objective: To determine the aflatoxins residue of animal medicines by immunoaffinity column HPLC method with post column photochemical derivatization and fluorescence detection, and to evaluate the feasibility of this method. The contamination status of aflatoxins in animal medicines was evaluated according to the determination data of aflatoxin contamination in animal medicines. Methods: After extraction by organic solvent and purification by immunoaffinity column, aflatoxins samples were analyzed by HPLC with fluorescence detection after photochemistry derivation. The recovery rates of aflatoxins in animal medicines, especially the species easily contaminated by aflatoxin, were then determined by adding aflatoxin standard mixtures. Finally, aflatoxins in animal medicines were determined and the results were analyzed. Results: Recovery rates of aflatoxin B1, B2, G1, and G2 were from 70% to 120%. Twenty-four batches of six kinds in 64 batches of 16 kinds of animal medicines were contaminated by aflatoxins, and the contamination rate was 37.5%. Thirteen batches of four kinds of animal medicines exceeded the limit of Chinese Pharmacopoeia of (2015 Edition), and the rate was 20.3%. Conclusion: This method can be used to determine aflatoxins in animal medicines. Some species of animal medicines are likely to be contaminated by aflatoxins, so the aflatoxin control in those animal medicines should be put forward to ensure the safety of drug use.