Analysis on chemical constituents in Tetrastigma hemsleyanum by UPLC-Triple - TOF/MS / 中草药

ABSTRACT

Objective: To establish a quick method of ultra-performance liquid chromatography-quadrupole time-of-fight mass spectrometry (UPLC-Triple-TOF/MS) for the analysis of compounds in Tetrastigma hemsleyanum. Methods: The separation was performed on the chromatographic column of Agilent Eclipse Plus-C18 (100 mm × 4.6 mm, 1.8 μm), and the mobile phase was methanol (0.1% formic acid)-0.1% formic acid solution, with a gradient elution at a flow rate of 1 mL/min. The column temperature was 30℃, and negative ion mode was used for TOF-MS. Results: Fifty-one compounds were identified or tentatively characterized based on the retention time and MS spectra. They are flavonoids, phenolic acids, phospholipids, glycolipids, benzenesulfoic acid, etc. And the preliminary fragmentation rules of phospholipid, glycolipids, and benzenesulfoic acid were summarized. Conclusion: The results demonstrate that UPLC-Triple-TOF/MS method is novel, quick, and efficient for the identification of the compounds in T. hemsleyanum.