Analysis on genetic diversity of germplasm resources in Pleione bulbocodioides from different habitats / 中草药

ABSTRACT

Objective: To analyze the genetic diversity and genetic relationship of 23 plant samples in Pleione bulbocodioides from different habitats. Methods: The molecular marker technique of inter-sample sequence repeat (ISSR) and unweighted pair-group method with arithmetic means (UPGMA) were used to study the genetic diversity and cluster analysis of 23 samples. Results: Twelve primers with diversity were sieved from 100 ISSR primers. DNA fragments (86) were amplified from 23 samples, and with 93.0% of polymorphic bands. In the cluster dendrogram, the studied samples were clustered into two groups, while the samples from the same region were in the same group and revealed the rule of geographical distribution in the tested materials. Conclusion: The study provides the foundation for identification and development of plants in P. bulbocodioides and guide collection and evaluation of germplasm resources.