Simultaneous determination of nine bufadienolides in Liushen Pill by HPLC-MS / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 700-705, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-852974
ABSTRACT
Objective:
To establish an HPLC-MS method for determining nine bufadienolides (gamabufotalin, arenobufagin, telocinobufagin, desacetylcinobufotalin, bufotalin, cinobufotalin, bufalin, cinobufagin, and resibufogenin) in Bufonis Venenum and Liushen Pill.Methods:
HPLC analysis was performed on ODS-2 column (250 mm×4.6 mm, 5 μm) with the mobile phase made up of acetonitrie and 0.15% phosphoric in water. Flow rate was set at 1 mL/min and column temperature was 40℃. The detection wavelength was 296 nm. Nine bufadienolides were determined by HPLC-ESI-TOF-MS with the mobile phase made up of acetonitrie and 0.2% formic acid in water. Eletrospray ionization source was applied and operated in the positive ion mode.Results:
Nine bufadienolides were separated at baseline with good linearity (r≥0.9996). The recovery rates were 92%-105% (RSD<5%).Conclusion:
The method established is rapaid, simple, and accurate, which is helpful to control the quality of Liushen Pill.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2017
Type:
Article
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