Effect of Bletilla striata polysaccharides on pharmacokinetics of 10 components in Panax notoginseng saponins / 中草药

ABSTRACT

Objective: To establish a UPLC-MS/MS method for simultaneous determination of 10 components in Panax notoginseng saponins (PNS), and the study the effect of Bletilla striata polysaccharides (BSP) on the pharmacokinetic parameters of PNS. Methods: Rats were divided into PNS group (P group) and PNS-BSP compatibility group (BP group) by ig administration. The plasma concentration of 10 saponins was determined by UPLC-MS/MS. The pharmacokinetic parameters were calculated by DAS software. Results: The determination method corresponded to the biological sample measurement requirements. Compared with P group, the AUC of Rb1 significantly reduced in BP group (P<0.01) and the AUCs of notoginsenoside R1, Rg1, Rf, Rd, CK, Rc, Rh1 were lower but without significant difference; The total AUC of 10 components significantly reduced in BP group (P<0.01); The plasma concentration of Rg1 in BP group was lower compared with that in P group (P<0.05); The tmax of Rg1, Rb1, and Rf significantly delayed (P<0.01); The tmax of notoginsenoside R1 and Rg2 delayed compared with P group (P<0.05). Conclusion: The established UPLC-MS/MS analysis method is suitable for the simultaneous determination of 10 components in PNS in rat plasma; PNS-BSP compatibility can reduce the plasma concentration of PNS and AUC exposure and prolong the tmax. This illustrates that BSP may increase the exposure levels of PNS in the gastrointestinal tract so that increase the effect in the gastrointestinal tract.