Determination of curcumin, desmethoxycurumin, and bisdesmethoxycurum in Curcuma longa by QAMS / 中草药

ABSTRACT

Objective: To establish a quality evaluation method for Curcuma longa, quantitative of multi-component with a single-marker (QAMS), to determine the contents of three curcuminoids at the same time. Methods: An HPLC method was used, and a Chromstar™ C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was methanol-4% glacial acetic acid (4852) at a flow of 1.0 mL/min. The detection wavelength was 422 nm and column temperature was 30℃. Curcumin was used as the internal reference substance. The relative correlation factors of curcumin, desmethoxycurumin, and bisdesmethoxycurumin to curcumin were calculated and evaluated by standard curve method and QAMS. Results: The QAMS method could be used to determine the contents of three curcuminoids at the same time, and with no significant difference between the results of standard curve method (RSD < 2.0%). Conclusion: QAMS method is simple, feasible, and reproducible, which could provide a reference for further research on completing the quality control of raw material and processed C. longa.