Separation process of three phenolic glycosides from Monochasma savatieri / 中草药

ABSTRACT

Objective: The optimal simultaneous separation process of three phenylethanoid glycosides (acteoside, isoacteoside, and torenoside B) from Monochasmasavatieri were established using of macrophage resins and dynamic axial column (DAC). Methods: The adsorption/desorption experiments and dynamic separation experiments were performed on eight types of resins (AB-8, D 101, HPD 100, LSA-10, LX-11, LX-17, LX-38, and XDA-6) to find the optimal resin. Then the optimal separation parameters were investigated on the chosen resin. The total phenylethanoid glycosides obtained from the large-scale experiment were further seperated to get acteoside, isoacteoside, and torenoside B, respectively using of DAC system. Results: Among these candidate resins, LX-17 was chosen to further obtain the optimal parameters The optimal feeding concentration of raw materials was 1.8 g/mL; The optimal adsorption time was 150 min; The optimal gradient elute conditions were ethanol/water (0/100, 4 BV; 15/85, 4 BV; 60/40, 5 BV; 90/10, 2 BV). The large-scale experiments were amplified to 10 folds on the basis of optimal parameters to obtain total phenylethanoid glycosides. Acteoside, isoacteoside, and torenoside B were simultaneously obtained from total phenylethanoidglycosides using DAC system. Conclusion: LX-17 and DAC system can be used for the purification of phenylethanoid glycosides, which will have a goodfuture for the application in industry.