Comparison on difference of ascorbic acid-glutathione cycle between wild and cultivated ginseng / 中草药

ABSTRACT

Objective: In order to explore the anti-oxidant capacity of wild and cultivated ginseng, the anti-oxidase activities and antioxidants contents on ascorbic acid-glutathione (AsA-GSH) cycle were compared. Methods: The activity of superoxide dismutase (SOD) was detected by NBT method and the activity of catalase (CAT) was detected by potassium permanganate titration. The anti-oxidase activities and anti-oxidants contents on AsA-GSH cycle were tested using spectrophotometric determination. The contents of glutamate (Glu), cysteine (Gly), and glycine (Cys) were tested by automatic amino acid analysis. Results: The activities of SOD and CAT in wild ginseng were higher than that of cultivated ginseng. In AsA-GSH cycle, the activities of anti-oxidase such as ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), and glutathione reductase (GR). And the contents of antioxidants such as glutathione (GSH), ascorbic acid (AsA), and dehydroascorbic acid (DHA) were also more in wild ginseng. And then the contents of amino acids (Glu, Gly, and Cys), the precursor of GSH synthesis were high expression in wild ginseng to keep the balance of AsA and GSH recirculation. Conclusion: The anti-oxidase activities and antioxidants content on AsA-GSH cycle in wild ginseng are higher than those in cultivated ginseng, which may be leaded to the antioxidant capacity of wild ginseng is stronger. It will provide a theoretical basis for efficacy differences research on wild and cultivated ginseng.