Effects of Astragalus-Angelica compatilibity on bone marrow hematopoiesis suppression induced by Cyclophosphamide in mice / 中草药

ABSTRACT

Objective: To investigate the effects of different proportion combinations of Astragalus and Angelica on bone marrow hematopoiesis suppression induced by Cyclophosphamide (CTX) in mice, and to analysis their interactions. Methods: The model of bone marrow hematopoietic function suppression in mice was established by ip injection of CTX. Recombinant human granulocyte-macrophage colony-stimulating factor (rhG-CSF) was used as the positive control drug, ICR mice in the drug groups were administered with the extracts of Astragalus, Angelica, and combinations of Astragalus and Angelica with different ratios (101, 51, 2.51, 11, 12.5, 15, and 110). To detect peripheral hemogram, the nucleated cell count in bone marrow (BMNC), the area of bone marrow hematopoietic tissue, spleen index (SI), as well as the contents of hematopoietic growth factor (HGF) in serum such as Erythropoietin (EPO), thrombopoietin (TPO), and Granulocyte-macrophage colony-stimulating factor (GM-CSF). To integrate all the test parameters using comprehensive index method, then to analyze the interaction between Astragalus and Angelica through multiple linear regression analysis. Results: Following continuous CTX injection for 3 d, peripheral hemogram significantly decreased, accompanied by GM-CSF and TPO contents declined, BMNC reduced, SI elevated, and bone marrow hematopoietic tissue area lessened from day 5 to day 7 after injection. Single Astragalus had no remarkable effects on peripheral hemogram, HGF contents, bone marrow hematopoietic tissue area and BMNC. Except for HGF and BMNC, Angelica alone could increase the numbers of WBC, RBC, and PLT in the peripheral blood, raise the area of bone marrow hematopoietic tissue. Astragalus mixed Angelica with the ratios of 51, 2.51, 11, 12.5, 15, and 110 could increase the numbers of WBC, RBC, and PLT along with HGF content, BMNC and bone marrow hematopoietic tissue area, while decreased SI. The comprehensive effect analysis displayed that the effects of Astragalus-Angelica with 11, 12.5, and 15 ratios on promoting hematopoiesis were strongest. The interaction analysis revealed Angelica played a greater role in advancing hematopoiesis than Astragalus did after being combined, meanwhile, the interaction of Astragalus combined with Angelica produced a nonnegligible positive effect on promoting hematopoiesis, namely synergism. Conclusion: Astragalus-Angelica compatilibity has the synergism on promoting hematopoiesis, which is better when the combination ratio of Astragalus and Angelica is 11, 12.5, or 15, furthermore, the effect of Angelica is greater than that of Astragalus.