Content of polysaccharide and 5-hydroxymethyl furfural (5-HMF) in Codonopsis Radix after cooked with rice and its impact on rabbit gastrointestinal smooth muscle in vitro / 中草药

ABSTRACT

Objective: The effect of Codonopsis Radix was significantly different before and after being cooked, and to study the content of polysaccharide and 5-hydroxymethyl furfural (5-HMF) in Codonopsis Radix after cooked with rice and its impact on rabbit gastrointestinal smooth muscle in vitro. Methods: Before and after being cooked, the content of polysaccharide and 5-HMF were determined with phenol sulfuric method and HPLC. BL-420F bio-functional experiment was applied to observe the impact of Codonopsis Radix processing with rice on rabbit gastrointestinal smooth muscle in vitro. Tests showed that acetylcholine (Ach), neostigmine, barium chloride (BaCl2), atropine, and adrenaline (Adr) intervened the relaxation of rabbit gastrointestinal smooth muscle in vitro. Results: The content of polysaccharide was significantly lower than pieces after cooked with rice, and the content of 5-HMF was significantly higher. After processing it can further excite spontaneous activity of gastrointestinal smooth muscle in vitro. It can enhance bowel neostigmine and BaCI2 caused intestinal excitement and tension, objecting Adr caused intestinal relaxation. Conclusion: 5-HMF was abundantly produced after Codonopsis Radix cooked with rice, caused excitability contraction on rabbit gastrointestinal smooth muscle in vitro, reproducing 5-HMF effect of gastrointestinal smooth muscle in vitro. It may be one of the material basis of Codonopsis Radix cooked with rice enhanced spleen. Its mechanism may be its collaborative control of N2R and βR results.