Study on HPLC fingerprint of Yinhuang Drop Pills / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 4370-4373, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-853095
ABSTRACT
Objective:
To establish the specific chromatogram of Yinhuang Drop Pills (YDP) by High performance liquid chromatography.Methods:
The chromatographic column Kromasil 100-5C18 (250 mm × 4.6 mm, 5 μm) was used, acetonitrile-0.4% phosphoric acid as mobile phase with gradient elution, and the detection wavelength was 372 nm. Through similarity evaluation software, the similarity of specific chromatogram of 10 batches YDP were calculated.Results:
The HPLC specific chromatogram of YDP showed 7 common peaks, of which 6 peaks (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid) from Lonicerae Flos, 1 common peaks (baicalin) from Scutellariae Radix; By comparing with the reference substances, identified 7 components, respectively, the neochlorogenic acid (peak 1), chlorogenic acid (peak 2), cryptochlorogenic acid (peak 3), 3,4-O-dicaffeoylquinic acid (peak 4), 3,5-O-dicaffeoylquinic acid (peak 5), 4,5-O-dicaffeoylquinic acid (peak 6), baicalin (peak 7). By the methodology validation, verified that this method has good precision, repeatability and stability, 10 batches of sample similarity is greater than 0.9.Conclusion:
This method was available for the quality control of YDP.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2016
Type:
Article
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