Determination of six flavonoids in Rhodiolae Crenulatae Radix et Rhizoma by HPLC-DAD / 中草药

ABSTRACT

Objective: To establish a determination method for the contents of ternatumoside II, rhodiosin, rhodionin, herbacetin, kaempferol, and rhodiolin in Rhodiolae Crenulatae Radix et Rhizoma and to compare the content differences of the six flavonid compounds in Rhodiolae Crenulatae Radix et Rhizoma from different sources. Methods: Using HPLC-DAD method and Kromasil C18 column (250 mm × 4.6 mm, 5 μm), with acetonitrile and water as mobile phase, gradient elution at flow rate of 1.0 mL/min and column temperature of 35℃. The detection wavelength was 382 nm. Results: Ternatumoside II, rhodiosin, rhodionin, herbacetin, kaempferol, and rhodiolin had good linearity in the ranges of 0.015 08-0.301 6, 0.123 2-2.464, 0.109 5-2.190 8, 0.007 8-0.156, 0.021 46-0.429 2, and 0.006 48-0.129 6 μg, respectively. The average recoveries of the six constituents were 98.17%-100.52% and RSD values were 1.64%-1.98%. Conclusion: The method is simple and accutate. It provides the reference for comprehensive quality control of Rhodiolae Crenulatae Radix et Rhizoma.