Determination of seven steroidal saponins in Paridis Rhizoma and polygerm varieties from different regions in Yunnan province by UPLC and establishment of fingerprint / 中草药

ABSTRACT

Objective: To establish a quantitative analysis method of seven steroidal saponins in Paridis Rhizoma and its polygerm varieties from different regions in Yunnan province, to simultaneously determine the contents of seven steroidal saponins, to establish the fingerprint for the polygerm varieties in Gaoligong Mountain by UPLC-ELSD, and to evaluate the qualities. Methods: It was detected with an Acquity UPLC® BEH C18 (2.1 mm × 50 mm, 1.7 μm) column, and gradually diluted with acetonitrile-water at a flow rate of 0.2 mL/min, drift tube temperature of 55℃, nitrogen pressure 275.8 kPa, and gain 500 by UPLC-ELSD. Results: The 23 samples could be separated and analyzed of seven steroids within 23 min. All the indexes of the methodological investigation met the requirements. The results showed that the contents of saponins from 19 samples met the requirement in Chinese Pharmacopoeia 2015. The average content of four saponins was 1.42% and among them the content from the sample in Gaoligong Mounta was up to 1.660%. The similarity was lower than 0.9 in the samples of Gaoligong Mountain, and there were eight common peaks in the fingerprints, which were identified four characteristic peaks. The type and content of seven saponins in the samples could not be clearly classified by PCA analysis and system cluster analysis. Conclusion: The method is convenient, accurate, and suitable for the quantitative analysis of Paridis Rhizoma and polygerm varieties. There is no obvious difference between Paridis Rhizoma and polygerm varieties in chemical components and contents. Paridis Rhizoma (polygerm varieties) in Gaoligong Mountain is a variety worth popularization and in-depth researches.