Study on flavonoids from Nicandra physaloides and their immune activities / 中草药

ABSTRACT

Objective: To investigate the flavonoids from the fruits of Nicandra physaloides and clarify the immune activities of the isolated compounds. Methods: The separations and purifications were taken by silica gel and ODS chromatogram columns as well as preparative HPLC, and the structural identification based on physicochemical property, 1H-NMR, and 13C-NMR as well as HR-MS data. And the immune activities were evaluated by mice splenic lymphocyte proliferation model induced by LPS. Results: Thirteen compounds were obtained from the butanol fraction of the 70% ethanol extract of N. physaloides fruits, which were identified as 3''-hydroxy-puerarin (1), puerarin-6″-O-glucoside (2), daidzein 8-C-[α-D-apiofranosyl-(1→6)]-β-D-glucopyranoside (3), puerarin (4), 3''-methoxyl-puerarin (5), chrysin 6-C-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside (6), oroxylin A-7-O-β-D-glucuronide methyl ester (7), daidzin (8), baicalin (9), quercetin-3-O-β-D-galactopyranoside (10), quercetin 3-rutinoside (11), kaempferol 3-rutinoside (12), and quercetin 3-glucoside (13), respectively. The results indicated that compounds 1-3, 7, 9-10, and 12-13 could inhibit the proliferation of mice spleen lymphocytes and 6 could promote the proliferation in the influence of mice spleen lymphocytes proliferation induced by LPS. Conclusion: Compounds 1-7 are isolated from the plants of Solanaceae for the first time, compounds 8-13 are obtained from the plants of Nicandra Adans. firstly. And the compounds 1-3, 6, 7, 9-10, and 12-13 possibly possess the potential immune activities.