Effect of SIRT1/NF-κB signal axis on delaying hematopoietic stem cell and progenitor cell senescence with ginsenoside R1 in aging model rat induced by D-galactose / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 4016-4020, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-853157
ABSTRACT
Objective:
To investigate the effect of SIRT1/NF-κB signal axis on delaying hematopoietic stem cell and progenitor cell senescence with ginsenoside Rg1 in ageing model rat induced by D-galactose.Methods:
Male SD rats (n = 40) aging from 6 to 8 weeks old were randomly divided into control group, aging model group, positive control group, Rg1 treated group, and Rg1 prevented group (n = 10). The aging rat model was prepared by sc D-galactose for continuous 42 d, then ginsenoside Rg1 was given in different time. After 2 d of the treatment, the Sca-1+ HSC/HPC was isolated by magnetic cell sorting (MACS). The changes of cells observed by senescence-associated β-galactosidase (SA-β-Gal) staining, cell cycle analysis and culture of mixed hematopoietic progenitor cell were used to investigate the treated aging effect of ginsenoside Rg1.The expression of senescence associated SIRT1, NF-κB mRNA and protein was examined by real time fluorescence quantitative PCR (FQ-PCR) and Western blotting.Results:
In ginsenoside Rg1 treated group and ginsenoside Rg1 prevented group, the percentage of positive cells expressed SA-β-Gal and the number of cells entering G0/G1 phase were lower than that of aging model group, but the number of CFU-Mix was increased than aging model group. Compared with aging model group, the expression of SIRT1 mRNA and protein was upregulated and the expression of NF-κB mRNA and protein was downregulated in Rg1 treated group and prevented group. Changes in Rg1 prevented group were more than those in Rg1 treated group.Conclusion:
SIRT1/NF-κB signal axis may play a key role in the anti-aging effect of Rg1 to Sca-1+ HSC/HPC senescence in ageing model rat induced by D-galactose.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS